Morphometrics and Molecular Characterization of New Isolate of Entomopathogenic Nematode, Heterorhabditis Bacteriophora Poinar, 1976 (nematoda, Rhabditida) from the North of Iran
نویسنده
چکیده
For the first time during the survey of entomopathogenic nematodes in north of Iran, a geographical isolates of Heterorhabditis recovered from soil using Galleria baiting technique. Based on morphological and molecular characterization this isolates was identified as Heterorhabditis bacteriophora NIR1. Morphometric and morphological comparisons of the new isolate with type species and other described isolates from different areas showed that H. bacteriophora NIR1 is morphologically similar to others but differ from type species in the following cases. For infective juvenile, means of body length (582 vs 570 μm), distance from anterior end to nerve ring (86 vs 83 μm) and esophagus length (121 vs 125 μm) and for male, means of body length (910 vs 820 μm), maximum body width (60 vs 43 μm), anal body width (27 vs 23 μm), esophagus length (114 vs 103 μm), distance from anterior end to excretory pore (155 vs 121 μm) and spicule length (42 vs 40 μm) were different from type isolate. Molecular studies of rDNA ITS regions showed that the Iranian isolate is closely related to the other described isolates of H. bacteriophora from other area. Sequence length of amplified region of ITS-rDNA of NIR1 isolate (819 base pairs) is the same as in type species. Intraspecific relationships among isolates of H. bacteriophora showed that geographic distribution is associated with the genetic and morphologic differences.
منابع مشابه
Characterization of an isolate of Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae) from the Northern Territory, Australia, using morphology and molecular data.
An entomopathogenic nematode, Heterorhabditis H39, was found in Darwin, Australia. Based on morphological and morphometric similarities, and molecular characterisation, it is an isolate of Heterorhabditis bacteriophora. Males, hermaphrodites, females and juveniles showed important similarities on most characters that define H. bacteriophora. The morphometrics of the infective juvenile of Hetero...
متن کاملEffects of pink bollworm resistance to Bacillus thuringiensis on phenoloxidase activity and susceptibility to entomopathogenic nematodes.
Widespread planting of crops genetically engineered to produce insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) imposes selection on many key agricultural pests to evolve resistance to Bt. Fitness costs can slow the evolution of Bt resistance. We examined effects of entomopathogenic nematodes on fitness costs of Bt resistance in the pink bollworm, Pectinophora gossypiella (Sau...
متن کاملINSECTICIDE RESISTANCE AND RESISTANCE MANAGEMENT Effects of Pink Bollworm Resistance to Bacillus thuringiensis on Phenoloxidase Activity and Susceptibility to Entomopathogenic Nematodes
Widespread planting of crops genetically engineered to produce insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) imposes selection on many key agricultural pests to evolve resistance to Bt. Fitness costs can slow the evolution of Bt resistance. We examined effects of entomopathogenic nematodes on Þtness costs of Bt resistance in the pink bollworm, Pectinophora gossypiella (Saun...
متن کاملSusceptibility of Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae) to Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae and Steinernematidae)
Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae) and entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) are two soil-dwelling biological control agents used to manage western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) and fungus gnats Bradysis spp. (Diptera: Sciaridae) in glasshouses. Growers often use multiple natural enemie...
متن کاملSurvival and Infectivity of the Insect-Parasitic Nematode Heterorhabditis bacteriophora Poinar in Solutions Containing Four Different Turfgrass Soil Surfactants
This laboratory study examined viability and infectivity of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora Poinar in solutions containing four different turfgrass soil surfactants: Revolution (Aquatrols Corp., Paulsboro, NJ), Aqueduct (Aquatrols Corp., Paulsboro, NJ), Cascade Plus (Precision Laboratories Inc., Waukegan, IL) and OARS (Aqua-Aid Inc., Rocky Mount, NC). Infective...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010